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Figure 4 | Journal of Experimental & Clinical Cancer Research

Figure 4

From: Improved retroviral suicide gene transfer in colon cancer cell lines after cell synchronization with methotrexate

Figure 4

Infection efficiency of the HSV- tk retroviral vector. DHDK12 cells (A) and HT29 cells (B) were treated for 24 hr with (filled square) or without (open square) MTX. Cells were transduced with TG 9344 at the indicated times after MTX washout. The HSV-TK expression level was determined 48 hr after transduction by flow cytometry using a mouse monoclonal 4C8 antibody against HSV-TK protein. Data are expressed as the mean ± SE from at least three separate experiments. *P <.05 vs. untreated cells, # P <.05 vs. MTX-treated cells at 12 and 16 hr after MTX withdrawal.

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