Table 1 Primers used in RNA expression
gene | Sequences | Tm (°C) | Product Size(bp) | |
|---|---|---|---|---|
QPCR | GAPDH | F:5'GGGAAACTGTGGCGTGAT3' R:5'GAGTGGGTGTCGCTGTTGA3' | 59 | 299 |
FHIT | F:5'GGAGATCAGAGGAGGAAATGG3' R:5'GGGAGTTGGAGTGACCGAG3' | 59 | 233 | |
PTEN | F:5'ACACGACGGGAAGACAAGTT3' R:5'CTGGTCCTGGTATGAAGAATG3' | 59 | 157 | |
CHFR | F:5'GCGTAGAAATGCCCAAACC3' R:5'TCCATCCAGCCCGAGTAGC3' | 59 | 171 | |
SFRP4 | F:5'GGCCTCTTGATGTTGACTGTAA3' R:5'GAGGGATGGGTGATGAGGA3' | 59 | 204 | |
PAX1 | F:5'GGTAGGAGTAGGGAGCACAGG3' R:5'CAAGTGTTGCGAGTGGAGG3' | 59 | 100 | |
TSLC1 | F:5'TTATTTCAGGGACTTCAGGC3' R:5'TTCCACCGCAGTGTCTTTC3' | 59 | 223 | |
CCNA1 | F:5'GCCTGGCAAACTATACTGTGAAC3' R:5'GTGCAGAAGCCTATGACGATTA3' | 59 | 295 | |