Figure 8
From: PinX1 regulation of telomerase activity and apoptosis in nasopharyngeal carcinoma cells
Effect of PinX1 on telomerase activity in nasopharyngeal carcinoma cells. Telomerase activity was measured by scratch assay in NPC 5-8 F cells (a) transfected with PinX1-FAM-siRNA, (b) untreated, (c) treated with lipofectamine alone, (d) transfected with pEGFP-C3, (e) transfected with pEGFP-C3-PinX1, and (f) human umbilical vein cells VEC-304. Lane (g) shows the DNA marker. The results indicate that telomerase activity is weak in ECV-304 and strong in untreated NPC 5-8 F cells and overexpression of PinX1 by transfection of pEGFP-C3-PinX1 significantly inhibited telomerase activity in NPC cells, but not affected by transfection of PinX1-FAM-siRNA and pEGFP-C3, and treatment with lipofectamine.