NSBP1 expression is high in ccRCC tissues and cells. (A), Representative immunohistochemistry staining of NSBP1 in control renal tissue and ccRCC tissue. NSBP1 immunoreactivity in brown was predominantly localized in the nucleus. Original magnification: X10 (a, c), X40 (b, d). (B) Ratio between protein expression levels of NSBP1 and β-Actin in pairs of ccRCC and normal tissue from 20 patients was calculated based on Western blot analysis. (C), Western blots demonstrating the expression of NSBP1 in different ccRCC cells. Actin served as loading control. (D), Real-time PCR assay showing the relative NSBP1 mRNA level in different ccRCC cells. *p < 0.05, **p < 0.01, versus HK-2 cells.