Figure 4

Characterizing OPN and its receptors in mouse NSCLCs. A Development of KPT model. Kras^G12D-LSLp53^fl/fl (KP) mice were inhaled with Adeno-CMV-Cre at approximately 8 weeks after birth. Lung tumors were inspected at approximately 18 weeks post-inhalation. Pieces of lung tumors were taken from transgenic mice and were implanted subcutaneously (without any in vitro manipulation) into Scid/beige mice using trocar to generate KPT (Kras^G12D-LSLp53^fl/fl trocar) model as described in the Materials and Methods. B Tumor implantation results in increased levels of OPN in the plasma in tumor bearing mice. C Using flowcytometry, expression of CD44v6 and αvβ3 was evaluated in KP cells and mPBMCs. Cells were stained with the antibodies as described in materials and methods and data analysis showed greater expression of αvβ3 than CD44 in both KP and mPBMCs. D KPT mice were randomized and received treatments (Vehicle, AOM1, Carboplatin and combination) at 8 days post-implantation. Tumors volume were measured twice/week and study was terminated at 27 days after implantation.