Figure 1

Pure curcumin down-regulated the expression of WT1 and inhibited the proliferation in K562 and HL-60 cells. (A and C) K562 cell was treated with non-cytotoxic doses of pure curcumin (5, 10, 20 uM) for 24 and 48 hours, then the mRNA level of WT1 was detected by qRT-PCR and the protein level of WT1 was detected by Western blotting after curcumin treatment for 48 hours. (B and D) HL-60 cell was treated with non-cytotoxic doses of pure curcumin (2.5, 5, 10 uM) for 24 and 48 hours, then the mRNA level of WT1 was detected by qRT-PCR and the protein level of WT1 was detected by Western blotting. GAPDH as loading control. (E and F) CCK8 assay was performed when K562 and HL-60 cells were treated for indicated concentration of curcumin for 24, 48 and 72 hours. ^# and *represent less than 0.05 and 0.01 of P-values, respectively, as compared to control.