Figure 3
A: The expression of HIF-1α mRNA in PC-2 cells treated with different concentration of CoCl 2 . 1. Normoxia group; 2. CoCl2 100 μmol/L group; 3. CoCl2 150 μmol/L group; 4. CoCl2 200 μmol/L group. This assay was done quintuplicate. Values represent means ± standard deviations (n = 5) and were determined using the Student's t-test. *P < 0.05 and **P < 0.01 versus Normoxia group. B: The expression of HIF-1α mRNA in PC-2 cells treated with 200 μmol/L CoCl2 for different time. 1. 0 h; 2. 4 h; 3. 8 h; 4. 12 h; 5. YC-1 2 h. This assay was done quintuplicate. Values represent means ± standard deviations (n = 5) and were determined using the Student's t-test. *P < 0.05, **P < 0.01 versus 0h, #P < 0.05 versus 12h. C: The expression of HIF-1α protein in PC-2 cells treated with different concentration of CoCl2. 1. Normoxia group; 2. CoCl2 100 μmol/L group; 3. CoCl2 150 μmol/L group; 4. CoCl2 200 μmol/L group. This assay was done quintuplicate. Values represent means ± standard deviations (n = 5) and were determined using the Student's t-test. *P < 0.05 and **P < 0.01 versus Normoxia group.