TGF-β induces EMT and enhances RGC-32 expression in BxPC-3 cells. BxPC-3 cells were cultured and treated with 10 ng/ml of TGF-β1 for 24 h, 48 h and 72 h, respectively. The morphology of cells at 72 h of TGF-β1 treatment was visualized with a phase contrast microscope (original magnification × 200, Nikon). (A) mRNA expression of E-cadherin, vimentin and RGC-32 was quantified by qRT-PCR with β-actin as an internal control. (B) Protein expression of E-cadherin, vimentin and RGC-32 was detected by western blot, (C) and normalized by β-actin (D). *P < 0.05 compared with the control group (0 h).