RGC-32 overexpression promotes EMT of BxPC-3 cells. BxPC-3 cells were transiently transfected with RGC-32 plasmid (pcDNA3.1/myc-His C-RGC32) or empty vector (pcDNA3.1/myc-His C). 72 h after transfection, qPCR (A) and western blot (B and C) were performed to examine the expression of RGC-32, E-cadherin and vimentin at mRNA and protein levels respectively. β-actin was used as an internal control. *P < 0.05.