Figure 3
GRP78 silencing inhibited the invasion and metastasis of SMMC7721. (A) Transwell analysis of the invasion capability of the cells that stably expressing shGRP78-3. The invaded cells were stained with Hochest33258 and observed using inverted fluorescent microscope, three fields were randomly chosen and the invasion capabilities of tumor cells were represented as the numbers of the invaded cells per field (scale bar: 25 μm). The experiments were repeated for three times. (B) Quantitative analysis of the invasive status of the cells that stably expressing shGRP78-3. The values were presented as ± SE and analyzed by one-way ANOVA; (Columns,mean of three separate experiments; bars, SE; *, values significantly different at the 5% levels). (C) Wound healing analysis of the metastasis of the cells that stably expressing shGRP78-3. The confluent cells were wounded by sterile pipettes and the status of wound closure were observed and photographed after 24 h.the experiment was repeated for three times. (scale bar: 25 μm) (D) Quantitative analysis of the metastasis status of the cells that stably expressing shGRP78-3. The values were presented as ± SE and analyzed by one-way ANOVA; (Columns,mean of three separate experiments; bars, SE; *, values significantly different at the 5% levels). (E) MTT analysis of the proliferation status of the cells that stably expressing shGRP78-3, the experiment was repeated for 3 times in tripilicate and The values were presented as ± SE and analyzed by one-way ANOVA; (Columns,mean of three separate experiments; bars, SE; *, values significantly different at the 5% levels).