Figure 4

GRP78 knockdown decreased ECM degradation. (A) FITC-gelatin degradation analysis of the extracellular matrix degradation capability of the cells that stably expressing shGRP78-3. The experiments were repeated for three times. (B) Western blot analysis of MMP-2,MMP-9,MMP-14 and TIMP-2 expression in the cells that stably expressing shGRP78-3, and the results of quantative analysis were represented as $\overline{x}$± SE and analyzed by one-way ANOVA (Columns,mean of three separate experiments; bars, SE; *, values significantly different at the 5% levels). (C) and (D) Gelatin zymograph analysis of the activities of MMP-2 and MMP-9 in GRP78 knockdown cells. The activities of MMP-2 and MMP-9 were represented as $\overline{x}$± SE and analyzed by one-way ANOVA (Columns,mean of three separate experiments; bars, SE; *, values significantly different at the 5% levels).