Figure 4

Sensitization to lysosomal membrane permeabilization and cell deathby LAMP1 shRNA. (A) Bxpc3 cells transformed with pLKO.1-LAMP1 or pLKO.1-Neg Ctl confirmed for knockdown of LAMP1/2 by flow cytometry. (B) Lysosomal retention of LysoTracker Green by flow cytometry of transformed cells treated with DMSO vehicle, SW43, or PB282 for one hour. Data represents mean fluorescence normalized to DMSO treated pLKO.1-Neg cells, n = 3. (C) Viability of transformed cells following 24 hour treatment with SW43, PB282, HCQ. Data represents percent viability compared to DMSO treated cells, n = 3, * p < 0.05.