Figure 6

Antioxidants are protective of cellular toxicity. (A) ROS detection by flow cytometry in Bxpc3 cells with 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) following 24 hour treatment with SW43 (60 μM), PB282 (90 μM), or hydrogen peroxide (H2O2, 100 μM) in the presence of lipophilic antioxidant α-tocopherol (α-toco) or hydrophilic antioxidant N-acetylcyteine (NAC). (B) Cell viability following 24 hour treatment with SW43 or PB282 in the presence of α-toco or NAC. Data represents percent viability compared to DMSO treated cells, n = 3, * p < 0.05.