Figure 4

T cell-stimulatory capacity of CD40-activated B cells. 1 x 10⁴ treated and control CD40-activated B cells were incubated with 1 x 10⁵ CFSE-labeled allogeneic T cells. After 5 days the proliferation of the allogeneic CD4⁺ and CD8⁺ T cells was assessed by flow cytometery. IL-10, TGF-β, or VEGF did not inhibit the proliferation of allogeneic CFSE-labeled CD4⁺ (n = 8) and CD8⁺ T cells (n = 5) in response to CD40-activated B cells. The data shown represents the means of the relative percentage of CD25⁺ proliferating, i.e. CFSE^low, T cells ± SD.