Figure 4

Expression of proteins associated with the PI3K/Akt signaling and the intrinsic (mitochondrial) apoptotic pathways after varying times of treatment of CA46 cells with baicalin. (A) Expression of p-Akt in various untreated cell types as detected by phospho-Akt specific antibody. Lane 1, CA46 cells; lane 2, Jurkat cells; lane 3, K562 cells; lane 4, HL-60 cells; lane 5, normal peripheral blood mononuclear cells-1; lane 6, normal peripheral blood mononuclear cells-2. (B-E) CA46 cells were treated with 40 μM baicalin for the times indicated. Protein expression was analyzed by Western blotting. (B) Western blot showing expression of β-actin, Akt, p-Akt, NF-κB, IκB, p-IκB, mTOR and p-mTOR. (C) Expression of p-Akt/Akt, NF-κB, IκB, p-IκB, and p-mTOR/mTOR relative to that of β-actin. (D) Western blot showing expression of β-actin, Bcl-2, Bax, cleaved caspase-9, cleaved caspase-3, and uncleaved (116 kD) and cleaved (85 kD) PARP. (E) Expression of cleaved caspase-9, cleaved caspase-3, uncleaved and cleaved PARP, Bax, and Bcl-2 relative to that of β-actin. Findings are representative of those obtained on three separate occasions. *P <0.05 compared to the 0 h control; ^†P <0.05 compared to 24 h treatment; ^‡P <0.05 compared to 48 h treatment.