Skip to main content
Figure 4 | Journal of Experimental & Clinical Cancer Research

Figure 4

From: Potential utility of eGFP-expressing NOG mice (NOG-EGFP) as a high purity cancer sampling system

Figure 4

In order to determine the cell viability, the cancer cells were cultured on dishes after FACS sorting and subsequently reimplanted into NOG-EGFP mice. A) Left panel (experimental): The fluorescent cells were invisible among the collected cancer cells cultured on the dishes under the fluorescent microscope. Right panel (control): Directly cultured cells from the xenografted TFK-1 tumors. Fluorescent cells were detectable in some areas under the fluorescent microscope. Black arrows indicate eGFP-expressing cells. B) TFK-1 cells cultured after FACS sorting were able to grow in the NOG-EGFP mice. Tumorigenicity of the sorted TFK-1 cells was directly compared with that of the unsorted TFK-1 cells shown in Figure 2A. A total amount of 5.0 × 105 cells was injected into each mouse (n = 6).

Back to article page