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Figure 3 | Journal of Experimental & Clinical Cancer Research

Figure 3

From: MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells

Figure 3

BIRC5 and LASP1 were identified as miR-203 target genes. (A) Immunoblots of BIRC5 and LASP1 protein in TNBC cells after treated with miR-203 precursor or control miRNA. β-actin was used as a loading control. (B) Relative BIRC5 and LASP1 expression at mRNA level in TNBC cells transfected with miR-203 precursor or control miRNA. The mRNA expression was normalized to that of β-actin. (C) Sequence alignment of miR-203 and its putative conserved target site in BIRC5 and LASP1 3’-UTR (downloaded from TargetScan 6.0). (D) Luciferase reporter assays of the interaction between miR-203 and the BIRC5 and LASP1 3’-UTRs. Assays were performed by co-transfection of miR-203 precursor with a luciferase reporter gene linked to the 3’-UTRs of BIRC5 and LASP1, containing either wild type or mutated miR-203 complementary sites. *, P < 0.05.

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