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Table 1 Sequences of Primers for MSP and RT-PCR

From: TGFBI promoter hypermethylation correlating with paclitaxel chemoresistance in ovarian cancer

Gene

Primer

Sequences

Temp (°C)

Length(bp)

TGFBI

M-F

5'-gAAAATTGAGTACGGGTATAGTGC-3'

58

162

 

M-R

5'-CCAAATTAAATAAACTACGAACGAA-3'

  
 

U-F

5'-AAAATTGAGTATGGGTATAGTGTGG-3'

56

161

 

U-R

5'-CCAAATTAAATAAACTACAAACAAA-3'

  
 

B-F

5'-TGGAAGTAGTTATAGGAGGTTTAAGG-3'

55

402

 

B-R

5'-CCCAAAACCAAAACCAAAAC-3'

  
 

qRT-F

5'-GGCTGCAGAGTCTGATGTGT-3'

55

81

 

qRT-F

5'-CGCTCACTTCCAGAGAGATG-3'

  

GAPDH

qRT-F

5'-GGACCTGACCTGCCGTCTAG-3'

55

99

 

qRT-R

5'-TAGCCCAGGATGCCCTTGAG-3'

  
  1. M: methylated primers [23], U: unmethylated primers [23], B: primers of BSP primers (designed using MethPrimer), qRT, primers of Quantitative Real-Time PCR.