Effects of 125I irradiation on gene methylation and mRNA expression in xenografts. (A) Relative expression of DNMT1 was detected using qRT-PCR. (B) Effects of 125I irradiation on gene methylation of BNIP3 and WNT9A in xenografts assayed by MeDIP-PCR. BNIP3 and WNT9A in treatment group displayed lower level of methylation when compared with control group. (C) Relative expression of BNIP3 and WNT9A was detected using qRT-PCR. Data are expressed as the mean ± SD of 6 samples. The significance of the varieties between the control group and 125I treatment group was analyzed through student’s t-t test. (☆: P < 0.05).