Figure 2

Prostaglandin receptors and cAMP and PLCβ responses. A) and B) Expression of prostaglandin receptor mRNA in MH₁C₁ cells (data from three experiments, measured in triplicate) and hepatocytes (data from one experiment measured in triplicate). Quantitative RT-PCR of EP1, EP2, EP3, EP4 and FP normalized to GADPH. RNA was isolated as described in Materials and Methods. * not detected # low levels-not quantifiable. C) Left: Accumulation of cAMP in MH₁C₁ cells after stimulation with either PGE₂ (100 μM) or isoproterenol (10 μM) in the presence of 0.5 mM IBMX. cAMP was measured after 3 minutes. Right: Accumulation of inositol phosphates in MH₁C₁ cells after stimulation with PGE₂ (100 μM) for 30 minutes in the presence of 15 mM LiCl. The data shown are mean ± S.E.M of three independent experiments.