Figure 3

Effect of different prostaglandin receptor inhibitors in MH ₁ C ₁ cells. A) The EP4 inhibitor L-161982 (10 μM) was added 30 min prior to stimulation with PGE₂ (100 μM) for 5 min. B) The EP1 inhibitor SC51322 (5 or 10 μM) was added 30 minutes prior to stimulation with PGE₂ (100 μM) for 5 min. C) The FP inhibitor AL8810 (10 or 100 μM) was added 30 minutes prior to stimulation with PGE₂ (100 μM) for 5 min. All blots are representative of three independent experiments. D) Effect of AL8810 (100 μM) on accumulation of inositol phosphates after stimulation with increasing concentrations of fluprostenol for 30 minutes in the presence of 15 mM LiCl. The data shown are mean ± S.E.M of four independent experiments.