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Figure 5 | Journal of Experimental & Clinical Cancer Research

Figure 5

From: Mechanisms involved in PGE2-induced transactivation of the epidermal growth factor receptor in MH1C1 hepatocarcinoma cells

Figure 5

Effect of Src and MMP inhibitors on phosphorylation of EGFR and downstream targets. A) MH1C1 cells were pretreated for 90 min with the Src inhibitor CGP 77675 (10 μM). Cells were then stimulated with either PGE2 (100 μM) or EGF (10 nM) for 5 min before they were harvested and immunoblotting performed as described in Materials and Methods. Representative blots of at least three experiments. B) MH1C1 cells were pretreated for 30 min with the Src inhibitor PP2 (10 μM). Cells were then stimulated with either PGE2 (100 μM) or EGF (10 nM) for 5 min before they were harvested and immunoblotting performed as described in Materials and Methods. Representative blots of two experiments. C) MH1C1 cells were pretreated for 30 min with increasing concentrations of the metalloproteinase inhibitor GM6001. Cells were then stimulated with PGE2 (100 μM) for 5 min before they were harvested and immunoblotting performed as described in Materials and Methods. Representative blots of three experiments D) MH1C1 cells were pretreated for 30 min with the metalloproteinase inhibitor GM6001 (10 μM). Cells were then stimulated with either PGE2 (100 μM) or EGF (10 nM) for five minutes before they were harvested and immunoblotting performed as described in Materials and Methods. Representative blots of at least three experiments E) Same experiment as in D) performed in hepatocytes. Representative blots of at least three experiments.

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