Figure 1

Induction of sCLU in a time and dose dependent fashion by gemcitabine treatment. A. Western analysis showing sCLU expression after 2–24 hours treatment with 0.5 nM gemcitabine. Induction of sCLU is evident in chemo-sensitive BxPC-3 cells when treated with high doses of gemcitabine but not in MIAPaCa-2, in which the high levels of sCLU remained unchanged. B. Western analysis showing sCLU expression in cell extracts after 12 hours treatment with 0.1-1.0 nM gemcitabine. sCLU increased in gemcitabine -sensitive BxPC-3 cells at different doses. At difference, expression of sCLU was unchanged in the MIAPaCa-2-resistant cells. The data shown are representative of three independent experiments.