Figure 2

Targeting sCLU by OGX-011 sensitizes pancreatic cancer cells to gemcitabine treatment. A, BxPC-3 and MIAPaCa-2 cells were transfected either with OGX-011 (1200nM) and then challenged with gemcitabine dose of 1.0 uM at 24 h. FACS analysis demonstrating that OGX-011 enhanced gemcitabine toxicity in both of the cells. B, Comparative viability of MIAPaCa-2 cells and BxPC-3 cells before and after sCLU sliencing. Cells were cultured in 96-well plates, then transfected either with OGX-011. Twenty-four hours after last transfection, cells were treated with gemcitabine. Seventy-two hours after drug addition ,cell viability was estimated. The data shown are representative of three independent experiments (*P < 0.05).