Figure 1

Hypoxia reduced HepG2 and MHCC97-H cell adhesion and facilitated invasion and migration. (A) An adhesion assay was performed with HCC cells on collagen I-coated plates. The relative cell adhesion number in each group is reflected in the column chart. The values of the normoxia-treated cells were set at 1. (B, C) Matrigel invasion assays of HepG2 and MHCC97-H cells were performed under normoxic and hypoxic conditions; the quantified data are shown in the diagram. (D, E) Transwell migration assays of HepG2 and MHCC97-H cells were performed under normoxic and hypoxic conditions; the numbers of cells are shown in the diagram. ^*, P < 0.05 compared to normoxia-treated HepG2 cells; ^†, P < 0.05 compared to normoxia-treated MHCC97-H cells. Original magnification: 200× (B, D).