Figure 2

Fluorescence in situ hybridization with gene, locus and centromeric specific probes. A) Neoplastic nuclei showing EGFR gene amplification (green signals) and polysomy of chromosome 7 (CEP7-orange signals); B) Representative case with normal EGFR gene status; C) MET high level gain (red signals) accompanied by high polysomy of chromosome 7 (CEP7-green signals); D) Normal ME T gene status, E) D7S486 locus deletion (red signals); F) D7S486 locus normal status. (Full size images X1000).