Figure 3

TheraScreen analysis of the KRAS gene in DNA isolated from NSCLC tissue. (A) Wild type. The figure shows a positive PCR control in which the quantity and quality of the DNA were assessed (red curve) without any other amplification of ARMS mutation positive primers (green curve) (B) Mutant. The figure shows a positive PCR control and a mutation signal (12Asp) generated by one tube of the ARMS-primers. The upper limit on ΔCt, which corresponds to a mutant DNA content of 1%, is for the mutant PCR to be 8 cycles behind the control PCR (here ΔCt = 26.44 - 24.03 = 2.41).