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Figure 6 | Journal of Experimental & Clinical Cancer Research

Figure 6

From: A comparison of Direct sequencing, Pyrosequencing, High resolution melting analysis, TheraScreen DxS, and the K-ras StripAssay for detecting KRAS mutations in non small cell lung carcinomas

Figure 6

Comparative sensitivity analysis of KRAS typing kits in dilution series, where DNA from three mutated cell lines was diluted in wild-type DNA. Results of dilution series consisted of 25%, 20%, 15%, 10%, 5%, 1%, 0.5%, 0.25%, and 0.125% of mutated DNA in wild-type DNA. For threshold found in the first dilution experiment and one adjacent concentration from each side, typing was performed three times. Resulting consensus thresholds (found two or three times out of three repeats) for cell lines A549 (p.Gly12Ser), NCI-H620 (p.Gly12Val), and NCI-H209 (p.Gly12Ala) are shown in the graph.

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