A comparison of Direct sequencing, Pyrosequencing, High resolution melting analysis, TheraScreen DxS, and the K-ras StripAssay for detecting KRAS mutations in non small cell lung carcinomas

Table 2 Number and percentage of mutations detected by methods

Methods	Mutations/samples	%	Mutations/samples	%
Direct sequencing	6/131	4.5	6/116	5.2
Pyrosequencing	10/131	7.6	10/116	8.7
HRM	-	-	15/116	13.1
TheraScreen DxS	20/131	15.2	17/116	14.6
K-ras StripAssay	26/131	19.8	24/116	20.7

To allow comparison with HRM, results are provided not only for 131 but also for 116 samples.

ISSN: 1756-9966