Effect of Ad-CALR/MAGE-A3 on anti-angiogenesis in vitro. Using matrigel coated 96 well plates, anti-angiogenesis ability was observed. (A) - (D): Photomicrographs showing representative views of tube formation assays. In the presence of Ad-CALR(C) or Ad-CALR/MAGE-A3(D), the number of connecting HUVEC was smaller than those of Null (A) and Ad-vector (B). Scale bars = 100 μm. (E): Bar represents the mean number of the cells per field. The tube formation assay showed that the transfection of Ad-CALR/MAGE-A3 attenuated the tube formation ability of HUVEC cells. Data are presented as mean ± SD (*P < 0.05, compared with HUVEC or HUVEC/Ad-VECTOR, P > 0.05, compared with HUVEC/Ad-CALR group).