Lymphangiogenesis in nonmetastatic sentinel lymph nodes. (A), (B) Double immunofluorescent images of CD45RB (green) and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1; red) in nonmetastatic sentinel lymph nodes (SLN). Increase in LYVE-1-positive lymphatic sinuses is evident in both subcapsular margins (A) and medulla (B). sm, subcapsular margins; Me, medulla; f, follicle; pc, paracortex. Scale bar = 50 μm. (C) Measurement of LYVE-1-positive lymphatic sinus area in control LNs and nonmetastatic SLNs. A significant increase was observed in non-metastatic SLNs, compared with untreated controls. Columns, mean; bar, standard error. *, P<0.001 relative to controls.