Correlation between Vascular endothelial growth factor C and Fms-related tyrosine kinase expressions in tumor-associated lymph nodes. (A) Expression of Vascular endothelial growth factor C (VEGF-C) mRNA detected by reverse transcription PCR in B16/F10 cells and tumor-bearing lymph nodes (LNs). Glyceraldehyde-3-phosphate dehydrogenase expression was used as a loading control. (B) Immunofluorescence image of VEGF-C expression in B16/F10 cells. Scale bar = 50 μm. (C)-(E) Double immunofluorescence images using antibodies specific for VEGF-C (green) and Fms-related tyrosine kinase (Flt-4; red) in primary melanoma of the tongue (C), tumor-bearing sentinel LNs (D), and LNs adjacent (aLN) to tumor-bearing LNs (E). Photographs show an increase in Flt-4-positive lymphatic vessels and sinuses. Scale bars = 50 μm.