Figure 2

Mesothelin re-expressing or mesothelin sliencing in pancreatic cancer cells. A, Whole-cell lysates from mesothelin shRNA-transfected pancreatic cancer cells were subjected to SDS-PAGE and immunoblotted with anti- mesothelin antibody. GAPDH was used as a loading control. B, RT-PCR analysis of total RNA (1 μg) isolated from vector control and mesothelin shRNA -transfected pancreatic cancer cells, GAPDH was used as a loading control. C, Whole-cell lysates from mesothelin cDNA -transfected pancreatic cancer cells were subjected to SDS-PAGE and immunoblotted with anti- mesothelin antibody. GAPDH was used as a loading control.