Figure 6

Effects of mesothelin on pancreatic cancer growth in the xenograft nude mouse model. A. Subcutaneous tumor volume of HPAC- mesothelin,Capan-2- mesothelin and MIA PaCa-2- mesothelin and their mock cells(2 × 10⁶)were subcutaneously inoculated into nude mice (8 mice per treatment group). Tumor size was measured weekly for 4 weeks. ^**p < 0.05,^*p>0.05. B. Subcutaneous tumor volume of AsPC-1-shRNA mesothelin, Capan-2-shRNA mesothelin and Capan-1-shRNA mesothelin (2 × 10⁶) were injected into the flank of nude mice (eight per treatment group). Tumor size was measured weekly for 4 weeks. ^**p < 0.05. C, Ki-67-positive cells were counted under ×400 magnifications in five randomly selected areas in each tumor sample. Mean ± SE of 8 tumor samples from individual mouse in each group. D, Mesothelin,P53,PUMA,bax and bcl-2 protein was detected by Western blot in tumor samples. E, Mesothelin regulates cell apoptosis in orthotopic pancreatic cancer tumors. TUNEL-positive cells were counted under ×400 magnifications in five randomly selected areas in each tumor sample. Mean±SE of 8 tumor samples from individual mouse in each group. F, Cleaved capase-3-positive cells were counted under ×400 magnifications in five randomly selected areas in each tumor sample. Mean ± SE of 8 tumor samples from individual mouse in each group.