Relative expression levels of miR-21, miR-128 and miR-342-3p in plasma from healthy controls and GBM patients, ROC curve analysis based on expression of each miRNA in plasma. (A, B, C) Expression levels of the miR-21, miR-128 and miR-342-3p are normalized to mmu-miR-295 and analyzed using 2-△△Ct method. Statistically significant differences were determined using the Mann–Whitney U test. Plasma levels of miR-21 are significantly higher in GBM samples than in control samples (P < 0.001), and levels of miR-128 and miR-342-3p are significantly lower in GBM samples than in control samples (P < 0.001). (B) The AUC for miR-21 was 0.9300 (95% CI: 0.7940-1.066) with 90.0% sensitivity and 100% specificity. (D,F) The AUC for miR-128 or miR-342-3p was 1.000 (95% CI: 1.000 – 1.000) with 90.0% sensitivity and 100% specificity.