Figure 2

MREs-regulated expression of exogenous gene in bladder cancer cells. (a) Expression of different miRNAs was detected in the pooled 14 bladder cancer and 8 normal bladder mucosal tissues. miRNA level in noncancerous bladder tissue was regarded as standard and U6 was selected as endogenous reference. Means ± SEM of three independent experiments were shown. (b) LuciferBMCase activity was quantified in T24 and RT-4 bladder cells as well as s that were transfected with luciferase reporter plasmids. The luciferase activity in these cells transfected with psiCheck2 was used as standard. Means ± SEM of three independent experiments were shown.