microRNA response elements-regulated TRAIL expression shows specific survival-suppressing activity on bladder cancer

Table 1 MiRNA response elements (MREs) for bladder cancer-specific downregulated miRNAs

miRNA	primer sequences
miR-1	Forward: 5′-TCGAGACAAACACCACATTCCA ACAAACACCACATTCCA ACAAACACCGC-3′
miR-1	Reverse: 5′-GGCCGCGGTGTTTGTTGGAATGT GGTGTTTGTTGGAATGT GGTGTTTGTC-3′
miR-99a	Forward: 5′-TCGAGACAAACACCTACGGGT ACAAACACCTACGGGT ACAAACACCGC-3′
miR-99a	Reverse: 5′-GGCCGCGGTGTTTGTACCCGTA GGTGTTTGTACCCGTA GGTGTTTGTC-3′
miR-101	Forward: 5′-TCGAGACAAACACCGTACTGT ACAAACACCGTACTGT ACAAACACCGC-3′
miR-101	Reverse: 5′-GGCCGCGGTGTTTGTACAGTAC GGTGTTTGTACAGTAC GGTGTTTGTC-3′
miR-133	Forward: 5′-TCGAGACAAACACCGGACCAAA ACAAACACCGGACCAAA ACAAACACCGC-3′
miR-133	Reverse: 5′-GGCCGCGGTGTTTGTTTTGGTCC GGTGTTTGTTTTGGTCC GGTGTTTGTC-3′
miR-218	Forward: 5′-TCGAGACAAACACCAAGCACAA ACAAACACCAAGCACAA ACAAACACCGC-3′
miR-218	Reverse: 5′-GGCCGCGGTGTTTGTTTGTGCTT GGTGTTTGTTTGTGCTT GGTGTTTGTC-3′
miR-490-5p	Forward: 5′-TCGAGACAAACACCATCCATG ACAAACACCATCCATG ACAAACACCGC-3′
miR-490-5p	Reverse: 5′-GGCCGCGGTGTTTGTCATGGAT GGTGTTTGTCATGGAT GGTGTTTGTC-3′
miR-493	Forward: 5′-TCGAGACAAACACCACCTTCA ACAAACACCACCTTCA ACAAACACCGC-3′
miR-493	Reverse: 5′-GGCCGCGGTGTTTGTTGAAGGT GGTGTTTGTTGAAGGT GGTGTTTGTC-3′
miR-517a	Forward: 5′-TCGAGACAAACACCTGCACGA ACAAACACCTGCACGA ACAAACACCGC-3′
miR-517a	Reverse: 5′-GGCCGCGGTGTTTGTTCGTGCA GGTGTTTGTTCGTGCA GGTGTTTGTC-3′

The underscored sequences indicated MREs of miR-1, miR-99a, miR-101, miR-133 and miR-218, miR-490-5p, miR-493 and miR-517a.

ISSN: 1756-9966