Figure 5
TNKS1 inhibition altered the expression of anti-apoptotic proteins. A, C. Western blot analysis of β-catenin, Cyclin D1, c-Myc and Bcl-2 proteins level in SH-SY5Y and SK-N-SH cells untransfected, XAV939 treatment, transfected with TNKS1 shRNA or control shRNA. β-actin was loading control. B, D. The bar graph showed mean ± SD of the ratio interest proteins/β-actin band intensity obtained by pooling the results from 3 independent experiments in SH-SY5Y and SK-N-SH cells respectively. CON: control group; XAV: XAV939 group; SCR: SCR group; shRNA: RNAi TNKS1 group. *P < 0.05, **P < 0.01 compared to controls.