The effect of LRIG1 transfection on expression of EGFR. A: The mRNA expression of LRIG1 was examined by real time-PCR after transfection. B: The mRNA expression of EGFR was examined by real time-PCR after transfection. C: The protein expression of LRIG1 and EGFR was examined by western blot after transfection. Upregulation of LRIG1 significantly decreased endogenous EGFR protein. D: Lysates were immunoprecipitated with rabbit anti-LRIG1 or control IgG and blotted with antibodies to EGFR or LRIG1 (*P < 0.05).