LRIG1 gene transfection induced apoptosis and inhibit invasion in bladder cancer cells. A: LRIG1 gene transfection induced apoptosis in human T24 and 5637 cell lines by flow cytometry analysis. B: The percentages are displyed showing the annexin V positive/7-aad negative fraction. Columns are expressed as mean ± SD of three independent experiments. *P < 0.01 for LRIG1 cDNA versus vector. C: Effect of LRIG1 gene transfection 24 h on the cell invasion of human bladder cancer cells. D: Data showed transfection of LRIG1 cDNA could significantly inhibit the cell invasion as compared with vector cells (*P < 0.05). All experiments were repeated at least three times.