Figure 1
Sorafenib inhibits the Raf/MEK/ERK signaling pathway. Human PDAC cells (AsPC-1, BxPC-3, Panc-1, MIA PaCa-2) were treated with sorafenib (So) (10 μM) for 4 hours. Total cell extracts were analyzed by immunoblotting for p-MEK (Ser221), total MEK, p-ERK1/2 (Thr202/Tyr204), total ERK, p-p70 S6K (Thr389), total p70 S6K, p-4E-BP1 and total 4E-BP1 proteins. Data are representative of two independent experiments with similar results.