Figure 3

Gemcitabine (Gem), sorafenib (So) and EMAP (E) inhibit in vitro cell proliferation of EC (HUVECs) and fibroblast cells (WI-38). Cells were plated on 96-well plate and treated with gemcitabine, sorafenib and EMAP. After 72 hours incubation, WST-1 reagent was added in each well and number of viable cells was calculated by measuring absorbance of color produced in each well. Data are representative of mean values ± SD of triplicate determinants. Symbols +, * and • represent p values of less than 0.05, 0.005 and 0.0005 compared to controls.