Figure 5

Forced-expression of SOX7 slows NSCLC proliferation . NSCLC cell lines (H23, H1299 and H1975) were stably infected and selected for stable expression of SOX7. (A) SOX7 vector uninfected (WT), GFP expression vector infected (GFP) or SOX7 expression vector infected SOX7 cells were confirmed in the three NSCLC cell lines by western blotting. β-actin was the control for equal loading. (B) Proliferation was measured by MTT assay. Each cell line was seeded in 96 well plates and absorbance was measured after 1, 2, 3 and 4 days culture. Results show the mean ±SD of quintuple wells. ** or ***, signifies statistical differences p < 0.01 or p < 0.001, respectively.