Figure 7

Forced-expression of SOX7 increases apoptosis in NSCLC by Annexin V-PI staining . Flow cytometry profile represents Annexin V-FITC staining in X-axis and propidium iodide in Y-axis. Dual staining of cells with Annexin V-APC and propidium iodide enabled categorization of cells into four regions. Region Q1 shows the necrotic cells, Q2 shows the late apoptotic cells, Q3 shows the live cells and Q4 shows the early apoptotic cells. Forced expression of SOX7 resulted in increase of early and late apoptotic cells in H23 and H1299 compared to GFP (control) cell. The figure is the representative of three independent experiments.