Figure 1

CpG inhibits cell proliferation and induces apoptotic death of A20.IIA lymphoma cells in vitro . (A) Flow cytometric analysis of TLR9 expression by A20.IIA cells after anti-TLR9 Ab staining (filled histogram), overlaid with isotype control (gray line). (B) CpG inhibits the proliferation of A20.IIA cells in vitro. 10⁴ A20.IIA cells were stimulated for 72 hours with various concentrations of CpG or control ODNs ranging from 0.0003 to 30μg/mL or with medium alone. The incorporation of the [³H] thymidine was measured by a scintillation counter. *P < 0.05; **P < 0.01. The data shown are representative of 1 of 3 experiments. (C) CpG induces apoptotic cell death of A20.IIA cell line. Cells were incubated for 72 hours with CpG or control ODNs at 3 and 30 μg/mL, or medium alone. The percentage of AnnV/PI positive cells was determined by flow cytometric analysis. ***P < 0.001.