Figure 2
From: Suppression of STIM1 inhibits human glioblastoma cell proliferation and induces G0/G1 phase arrest
Effect of STIM1 silencing on U251 cell proliferation. (A) Cell proliferation of lentivirus-transduced and TRPC entryway paralysed U251 cell were measured by MTT assay once daily. Cell proliferation was expressed as the absorbance values. (B) DNA synthesis was measured by BrdU incorporation assay at 24 h and 72 h after transduction. Growth rate (R) was calculated by the following equation to reflect the role of STIM1 in DNA synthesis ability: R = (A72h - A24h) / A24h × 100 where A72h and A24h indicate the absorbance at 450 nm after 24 and 72 hrs of incubation. (C), (D) Detection of cell proliferation by plate colony formation assay in U251 and U373cells. Representative photographs showing U251 and U373 cell colony in 6-well plate. U251 and U373 cells were seeded at 200 per well and allowed to form colonies. Cell colonies were scored visually and counted using a light microscopy. Data represent the mean ± S.D. of three independent experiments. **P < 0.01 compared with the si-CTRL group. si-CTRL: cells infected with control-siRNA-expressing lentivirus; si-STIM1: cells infected with si-STIM1.