Figure 4
From: Suppression of STIM1 inhibits human glioblastoma cell proliferation and induces G0/G1 phase arrest
mRNA and protein levels of cell cycle-related molecules. (A) Total RNA was extracted at 72 hrs after transduction and mRNA expression of p21Waf1/Cip1, cyclin D1 and CDK4 was determined by quantitative real-time RT-PCR. GAPDH was used as an internal control. (B) Total cellular protein were extracted at 72 hrs after transduction and determined by Western blot analysis using antibodies against p21Waf1/Cip1, cyclin D1 and CDK4, and GAPDH as a loading control. Data represent the mean ± S.D. of three independent experiments. *P <0.05, **P < 0.01 compared with the si-CTRL group. si-CTRL: cells infected with control-siRNA-expressing lentivirus; si-STIM1: cells infected with si-STIM1.