Figure 5

Effect of STIM1 knockdown on tumorigenicity in nude mice. U251 cells transduction with si-STIM1 or siCTRL were subcutaneously injected into the right dorsal flank of the nude mice as described in Materials and methods. Tumor volume was determined on Day 10, 20 and 30. At the end of the experiment, animals were sacrificed and tumors were excised for weight measurement and Western blot analysis. (A) Growth curve of tumor xenografts was assessed by serial microcaliper measurements. (B) Weight of tumor xenografts 30 days after inoculation. (C) Representative photographs of mice and tumors for each treatment. (D) Total protein from the resected tumors were prepared and used to determine the expression of STIM1 by Western blot analysis using antibodies against STIM1 and GAPDH. Data represent the mean ± S.D. of three independent experiments. *P <0.05, **P < 0.01 compared with the si-CTRL group. si-CTRL: cells infected with control-siRNA-expressing lentivirus; si-STIM1: cells infected with si-STIM1.