Figure 4

Effects of the caspase inhibitor Z-VAD-FMK on apoptosis induced by HU331 and V in human melanoma M14 cells. Z-VAD-FMK (30 μM) was administered 30 min before incubation with HU-331 and V (10 μM) for 72 h and the % of apoptotic cell was evaluated by flow cytometry (mean ± SEM of three experiment performed in triplicate; ***P < 0.001 vs control cells, §§§ P < 0.001 HU331 vs V treated cells.