Table 1 The primers and primary antibody used in this study
Gene symbles | Primers for RT-PCR | Â | Antibodies for IHC | Â | Â | |
|---|---|---|---|---|---|---|
|  |  | Primer sequences | Annealing temperature (°C) | Antibody sources | Clone | Dilution |
Bmi1 | Reverse | 5’-ATT GTC TTT TCC GCC CGC TT-3’ | 58.2 | ProMab Biotechnologies Inc | 3E3 | 1:800 |
Forward | 5’-TGG CAT CAA TGA AGT ACC CTC-3’ | |||||
CD44 | Reverse | 5’-TGC TAC TGA TTG TTT CAT TGC G-3’ | 56.2 | ProMab Biotechnologies Inc | 8E2F3 | 1:30000 |
Forward | 5’-GGA CCA GGC CCT ATT AAC CC-3’ | |||||
CD133 | Reverse | 5’-AAA CAA TTC ACC AGC AAC GAG-3’ | 54.1 | ProMab Biotechnologies Inc | 3 F10 | 1:400 |
Forward | 5’-TAG TAC TTA GCC AGT TTT ACC G-3’ | |||||
Sox2 | Reverse | 5’- GCT AGT CTC CAA GCG ACG AA-3’ | 56.2 | ProMab Biotechnologies Inc | 10 F10 | 1:800 |
Forward | 5’- TAC AGT CTA AAA CTT TTG CCC TT-3’ | |||||
Nanog | Reverse | 5’-AGG CAA CTC ACT TTA TCC CAA-3’ | 54.1 | Cell signaling technology | D73G4 | 1:300 |
Forward | 5’-GAT TCT TTA CAG TCG GAT GCT T-3’ | |||||
Oct-4 | Reverse | 5’-TGC AGA AAG AAC TCG AGC AA-3’ | 56.2 | Santa Cruz Biotechnology | C-10 | 1:50 |
Forward | 5’-CTC ACT CGG TTC TCG ATA CTG G-3’ | |||||
Msi2 | Reverse | 5’-CAG ACC TCA CCA GAT AGC CTT-3’ | 56.2 | ProMab Biotechnologies Inc | 2C11 | 1:1000 |
Forward | 5’-TAC TGT GTT CGC AGA TAA CCC-3’ | |||||
β-actin (217 bp) | Reverse | 5’GTG ACG TGG ACA TCC GCA AAG-3’ | 60.2 |  |  |  |
Forward | 5’-ATC CAC ATC TGC TGG AAG GTG GAC-3’ |  |  |  | ||
β-actin (417 bp) | Reverse | 5’-ACA GAG CCT CGC CTT TGC CGA TC-3’ | 60.2 |  |  |  |
|  | Forward | 5’-TGG GTC ATC TTC TCG CGG TTG G-3’ |  |  |  |  |